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pacific blue conjugated mouse anti dog cd4  (Bio-Rad)


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    Structured Review

    Bio-Rad pacific blue conjugated mouse anti dog cd4
    Facs sorted CD11b + CD14 − MHCII − cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated <t>CD4</t> + and CD8 + T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3 H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.
    Pacific Blue Conjugated Mouse Anti Dog Cd4, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pacific blue conjugated mouse anti dog cd4/product/Bio-Rad
    Average 93 stars, based on 23 article reviews
    pacific blue conjugated mouse anti dog cd4 - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer"

    Article Title: Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0033274

    Facs sorted CD11b + CD14 − MHCII − cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4 + and CD8 + T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3 H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.
    Figure Legend Snippet: Facs sorted CD11b + CD14 − MHCII − cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4 + and CD8 + T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3 H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.

    Techniques Used: Isolation, Incubation, Negative Control



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    93
    Bio-Rad pacific blue conjugated mouse anti dog cd4
    Facs sorted CD11b + CD14 − MHCII − cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated <t>CD4</t> + and CD8 + T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3 H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.
    Pacific Blue Conjugated Mouse Anti Dog Cd4, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pacific blue conjugated mouse anti dog cd4/product/Bio-Rad
    Average 93 stars, based on 1 article reviews
    pacific blue conjugated mouse anti dog cd4 - by Bioz Stars, 2026-03
    93/100 stars
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    Facs sorted CD11b + CD14 − MHCII − cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4 + and CD8 + T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3 H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.

    Journal: PLoS ONE

    Article Title: Identification of Myeloid Derived Suppressor Cells in Dogs with Naturally Occurring Cancer

    doi: 10.1371/journal.pone.0033274

    Figure Lengend Snippet: Facs sorted CD11b + CD14 − MHCII − cells isolated from a dog with osteosarcoma or healthy PBMCs were co-incubated with mitogen-stimulated CD4 + and CD8 + T cells isolated from a healthy dog for 72 hs. No stimulated cells were used as negative control. Proliferative responses were measured by 3 H-thymidine incorporation from experiments performed in triplicate. CPM, counts per minute. Mean ± SEM are shown.

    Article Snippet: For T cell isolation, PBMCs were isolated as previously described from fresh blood samples of healthy dogs and stained with 0.3 μg of FITC-conjugated mouse anti-dog CD3 (clone CA17.2A12, AbD Serotec), 0.15 μg of Pacific blue-conjugated mouse anti-dog CD4 (clone YKIX302.9, AbD Serotec) and 0.15 μg of Alexa700-conjugated mouse anti-dog CD8 (clone YCATE55.9, AbD Serotec) antibodies.

    Techniques: Isolation, Incubation, Negative Control